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epi fluorescence microscope  (Nikon)


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    Structured Review

    Nikon epi fluorescence microscope
    Epi Fluorescence Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 11795 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/epi+fluorescence+microscope/ECLIPSE+Ti2/bio_rxiv__64898__2026__03__04__709609-81-14-18
    Average 99 stars, based on 11795 article reviews
    epi fluorescence microscope - by Bioz Stars, 2026-09
    99/100 stars

    Images

    Related Articles

    Microscopy:

    Article Title: Volumetric localization microscopy with deep learning
    Article Snippet: .. The high-resolution Fourier light-field microscopy system (Supplementary Fig. ) was developed using an epi-fluorescence microscope (Eclipse Ti2-U, Nikon Instruments) . .. Briefly, an oil-immersion objective lens featuring 100× magnification and 1.45 numerical aperture (CFI Plan Apochromat Lambda 100× Oil, Nikon Instruments) was used.

    Article Title: Multiscale microfluidic platform with vacuum-driven chambers for automated high-volume ssDNA generation.
    Article Snippet: We present a multiscale microfluidic platform for automated high-volume single-stranded DNA (ssDNA) generation through plasmonic bead-based PCR.. Central to the system are vacuum-driven chambers capable of handling fluid volumes in the tens of microliters—at least an order of magnitude larger than conventional microfluidic chambers.. These chambers are seamlessly integrated with microfluidic components such as micromixers and pneumatically actuated microvalves, enabling precise fluidic transport in processing high volumes.

    Article Title: Myosin II regulates cellular thermo-adaptability and the efficiency of immune responses.
    Article Snippet: .. Brightfield imaging of the migration of RAW 264.7 and Jurkat T-cells was performed on an epi-fluorescence microscope (Nikon Eclipse Ti2, Tokyo, Japan) using a 10x Plan Fluor objective (NA = 0.3). .. The microscope was further equipped with a cage incubator (Okolab, Shanghai, China) for temperature and CO 2 control, a sCMOS camera (Zyla 4.2, Andor, Northern Ireland), and a x-y-stage (CMR-STG-MHIX2-motorized table, Mä rzhä user, Germany).

    Article Title: Real-time, high-throughput super-resolution microscopy via panoramic integration
    Article Snippet: .. The system was constructed using an epi-fluorescence microscope (Nikon Eclipse Ti2-U) equipped with a 100×, 1.45 NA oil-immersion objective lens (Nikon CFI Plan Apochromat Lambda 100× Oil), following established configurations , , . .. For excitation, a 488-nm laser (Coherent OBIS LX) generated multifocal illumination, which was spatially modulated by a microlens array (MLA; RPC Photonics MLA-s100-f4-A-R1; 100-μm pitch, F-number 4.2).

    Article Title: Uridine analogs prevent stress granule formation, not by blocking PKR recognition, but by inhibiting the synthesis of T7 RNA Polymerase byproducts.
    Article Snippet: Lastly, the coverslips were mounted onto glass coverslips using DAPI Fluoromount-G stain mounting solution (Southern Biotech). .. Microscopy and image analysis Cells were imaged using an epi-fluorescence microscope with a 60x objective (Nikon). .. Image analysis of stress granule presence and eIF2α phosphorylation levels were performed using Nikon Imaging Software (NIS) Elements Advanced Research (Nikon).

    Article Title: Microparticle separation based on size, density and shape in a sharp-corner MOFF channel using hydrodynamic effects
    Article Snippet: .. An EPI-fluorescence microscope (Nikon Eclipse LV100, Nikon, Japan) with a 20× objective (CFI60 TU Plan Epi ELWD, NA = 0.4) is used for the optical measurements. ..

    Article Title: PAVR: High-Resolution Cellular Imaging via a Physics-Aware Volumetric Reconstruction Framework
    Article Snippet: .. The PAVR imaging experiments were performed on a previously developed high-resolution FLFM using an epi-fluorescence microscope (Eclipse Ti2-U, Nikon Instruments) . .. In the microscope system, we implemented a 100× oil-immersion objective lens with a numerical aperture of 1.45 (CFI Plan Apochromat Lambda 100× Oil, Nikon Instruments) and a piezo nano-positioner (Nano-F100S, Mad City Labs) for precise positioning.

    Biomarker Discovery:

    Article Title: Multiscale microfluidic platform with vacuum-driven chambers for automated high-volume ssDNA generation.
    Article Snippet: We present a multiscale microfluidic platform for automated high-volume single-stranded DNA (ssDNA) generation through plasmonic bead-based PCR.. Central to the system are vacuum-driven chambers capable of handling fluid volumes in the tens of microliters—at least an order of magnitude larger than conventional microfluidic chambers.. These chambers are seamlessly integrated with microfluidic components such as micromixers and pneumatically actuated microvalves, enabling precise fluidic transport in processing high volumes.

    Fluorescence:

    Article Title: Multiscale microfluidic platform with vacuum-driven chambers for automated high-volume ssDNA generation.
    Article Snippet: We present a multiscale microfluidic platform for automated high-volume single-stranded DNA (ssDNA) generation through plasmonic bead-based PCR.. Central to the system are vacuum-driven chambers capable of handling fluid volumes in the tens of microliters—at least an order of magnitude larger than conventional microfluidic chambers.. These chambers are seamlessly integrated with microfluidic components such as micromixers and pneumatically actuated microvalves, enabling precise fluidic transport in processing high volumes.

    Imaging:

    Article Title: Multiscale microfluidic platform with vacuum-driven chambers for automated high-volume ssDNA generation.
    Article Snippet: We present a multiscale microfluidic platform for automated high-volume single-stranded DNA (ssDNA) generation through plasmonic bead-based PCR.. Central to the system are vacuum-driven chambers capable of handling fluid volumes in the tens of microliters—at least an order of magnitude larger than conventional microfluidic chambers.. These chambers are seamlessly integrated with microfluidic components such as micromixers and pneumatically actuated microvalves, enabling precise fluidic transport in processing high volumes.

    Article Title: Myosin II regulates cellular thermo-adaptability and the efficiency of immune responses.
    Article Snippet: .. Brightfield imaging of the migration of RAW 264.7 and Jurkat T-cells was performed on an epi-fluorescence microscope (Nikon Eclipse Ti2, Tokyo, Japan) using a 10x Plan Fluor objective (NA = 0.3). .. The microscope was further equipped with a cage incubator (Okolab, Shanghai, China) for temperature and CO 2 control, a sCMOS camera (Zyla 4.2, Andor, Northern Ireland), and a x-y-stage (CMR-STG-MHIX2-motorized table, Mä rzhä user, Germany).

    Article Title: PAVR: High-Resolution Cellular Imaging via a Physics-Aware Volumetric Reconstruction Framework
    Article Snippet: .. The PAVR imaging experiments were performed on a previously developed high-resolution FLFM using an epi-fluorescence microscope (Eclipse Ti2-U, Nikon Instruments) . .. In the microscope system, we implemented a 100× oil-immersion objective lens with a numerical aperture of 1.45 (CFI Plan Apochromat Lambda 100× Oil, Nikon Instruments) and a piezo nano-positioner (Nano-F100S, Mad City Labs) for precise positioning.

    Migration:

    Article Title: Myosin II regulates cellular thermo-adaptability and the efficiency of immune responses.
    Article Snippet: .. Brightfield imaging of the migration of RAW 264.7 and Jurkat T-cells was performed on an epi-fluorescence microscope (Nikon Eclipse Ti2, Tokyo, Japan) using a 10x Plan Fluor objective (NA = 0.3). .. The microscope was further equipped with a cage incubator (Okolab, Shanghai, China) for temperature and CO 2 control, a sCMOS camera (Zyla 4.2, Andor, Northern Ireland), and a x-y-stage (CMR-STG-MHIX2-motorized table, Mä rzhä user, Germany).

    Construct:

    Article Title: Real-time, high-throughput super-resolution microscopy via panoramic integration
    Article Snippet: .. The system was constructed using an epi-fluorescence microscope (Nikon Eclipse Ti2-U) equipped with a 100×, 1.45 NA oil-immersion objective lens (Nikon CFI Plan Apochromat Lambda 100× Oil), following established configurations , , . .. For excitation, a 488-nm laser (Coherent OBIS LX) generated multifocal illumination, which was spatially modulated by a microlens array (MLA; RPC Photonics MLA-s100-f4-A-R1; 100-μm pitch, F-number 4.2).



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    Nikon ti e inverted epi fluorescence microscope
    Electrospun nanofibers and nanorods from macroscopic to nanoscopic scale. (A) Samples of fiber mats obtained in 35 mm dishes under white light (B) Samples of fiber mats obtained in 35 mm dishes under UV lamp (365 nm) (C) Fiber mat loaded with R18 dye during electrospinning process. Cylindrical collector is 20 cm length with 10 cm diameter. (D,E) <t>Respective</t> <t>epifluorescence</t> images of BlueCy/PtOEP and R18/Pt‐TPTBP fibermats with 2× objective (scale bar 1 mm). Insert show higher magnification with 60x objective (scale bar 50 µm). (F,G) TEM micrographs of BlueCy/PtOEP (F) and R18/Pt‐TPTBP (G) nanorods. (H,I) Scale bar: 500 nm. Diameter distribution of BlueCy/PtOEP (H) and R18/Pt‐TPTBP (I) nanorods measured from TEM images.
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    Image Search Results


    Electrospun nanofibers and nanorods from macroscopic to nanoscopic scale. (A) Samples of fiber mats obtained in 35 mm dishes under white light (B) Samples of fiber mats obtained in 35 mm dishes under UV lamp (365 nm) (C) Fiber mat loaded with R18 dye during electrospinning process. Cylindrical collector is 20 cm length with 10 cm diameter. (D,E) Respective epifluorescence images of BlueCy/PtOEP and R18/Pt‐TPTBP fibermats with 2× objective (scale bar 1 mm). Insert show higher magnification with 60x objective (scale bar 50 µm). (F,G) TEM micrographs of BlueCy/PtOEP (F) and R18/Pt‐TPTBP (G) nanorods. (H,I) Scale bar: 500 nm. Diameter distribution of BlueCy/PtOEP (H) and R18/Pt‐TPTBP (I) nanorods measured from TEM images.

    Journal: Advanced Healthcare Materials

    Article Title: Fluorescent Polymeric Nanofibers as Ratiometric Multiplexed Skin Sensors of pH and Oxygen

    doi: 10.1002/adhm.202502717

    Figure Lengend Snippet: Electrospun nanofibers and nanorods from macroscopic to nanoscopic scale. (A) Samples of fiber mats obtained in 35 mm dishes under white light (B) Samples of fiber mats obtained in 35 mm dishes under UV lamp (365 nm) (C) Fiber mat loaded with R18 dye during electrospinning process. Cylindrical collector is 20 cm length with 10 cm diameter. (D,E) Respective epifluorescence images of BlueCy/PtOEP and R18/Pt‐TPTBP fibermats with 2× objective (scale bar 1 mm). Insert show higher magnification with 60x objective (scale bar 50 µm). (F,G) TEM micrographs of BlueCy/PtOEP (F) and R18/Pt‐TPTBP (G) nanorods. (H,I) Scale bar: 500 nm. Diameter distribution of BlueCy/PtOEP (H) and R18/Pt‐TPTBP (I) nanorods measured from TEM images.

    Article Snippet: Microscopy imaging was performed with a Nikon Ti‐E inverted epi‐fluorescence microscope, equipped with following objectives: CFI Plan Apochromat Lambda D 2X (NA = 0.1), CFI Plan Fluor 10X (NA = 0.3), CFI Plan Apochromat Lambda D 20X (NA = 0.75), CFI Plan Apo × 60 oil (NA = 1.4), CFI Apo TIRF 100XC Oil (NA = 1.49) and a Hamamatsu Orca Flash 4 sCMOS camera.

    Techniques: